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screening of anticancer plant extracts using culture

Medicinal Plants: Their Use in Anticancer Treatment - PMC

The plant extracts induced apoptosis which was demonstrated by an increased sub-G 1 phase population of cells ... preserving biological material in liquid nitrogen and tissue culture; propagates plants in sterile conditions and can produce mature plants ... In vitro anticancer screening of South African plants. Journal of Ethnopharmacology. ...

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Characterization of phytochemicals and evaluation of anti-cancer ...

The aim of the present study was to evaluate the in vitro anti-cancer and anti-oxidant potential of methanol extract of Blumea eriantha as well as its phytochemical characterization. The in vitro cytotoxic and antiproliferative activities of B. eriantha methanolic extract of leaves were evaluated using MTT assay on HeLa and B16F10 cell lines, and wound scratch and colony formation assays on ...

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IIUM: Staff Directory - International Islamic University Malaysia

His current research is on wound healing, anti-cancer, anti-obesity and anti-diabetes study of several medicinal plants using animal cell culture. He has published several papers in reputable journals and has contributed chapters in books. ... Study on Effects of Plant Extracts on Glucose Uptake in Cultured Adipocyte:

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Thymol screening, phenolic contents, antioxidant and antibacterial ...

3 days agoThe reason for using synthetic thymol was due to comparing it with high thymol content natural studied EO and extracts. Since the plant extract contained flavonoids and phenolic acids, it was ...

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Antimicrobial Activity and Phytochemical Analysis of Endophytic Fungal ...

Five mm cylindrical pieces cut out from well grown culture of the endophytic fungi were placed on Petri dishes swabbed with the test microorganisms, incubated at 2 - 8ºC for 12hrs to allow the diffusion of antimicrobial substance. Thereafter, bacterial plates were incubated at 37ºC for 24hrs and Yeast plates were incubated at 25ºC for 48hrs.

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Plants | Free Full-Text | Phytochemical Profiling and Assessment of ...

Plant cell culture is a source of plant material from which bioactive metabolites can be extracted. In this work, the in vitro propagation of Leptocarpha rivularis, an endemic Chilean shrub with anticancer activity, is described. Different media were tested and optimized for the introduction, propagation, and rooting steps of the micropropagation process. At the end of this process, 83% of ...

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ANTICANCER EFFECT AND APOPTOSIS INDUCTION OF Cymbopogon Citratus PLANT ...

MTT assay is a simple and reliable technique to measure the cell viability for screening of anti-proliferative agents. Treatment with the essential oil in HTB43 cancer cells resulted in growth inhibition with IC50 = 15.42, 6.10 ., Apoptosis induction of lemongrass essential oil treated HTB43 cancer cells,

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Antiproliferative effect of extracts and fractions of the root of ...

The study was directed to appraise the cytotoxic activity of the different extracts of T.avicennioides on normal Vero monkey kidney and human hepatocellular carcinoma HepG2 cells. Plants contain a variety of phytoconstituents that may not have direct effect on the plants' growth [] but which are assumed to have biologic activities.The phytoconstituents in the extract and fractions of the ...

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A Review on Pharmacognostical Phytochemical and Ethnomedicinal ...

Hedychium coronarium J. Koenig is a perennial medicinal herb which is distributed in throughout the world and used as traditional medicine since the ancient time. It contains various bioactive compounds including Phenols, Terpenoids, Saponins, Volatile oils, Flavonoids, Glycosides etc. These bioactive compounds made this herb as a valuable potent herbal drug. Hedychium coronarium has high ...

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Ecology‐based screen identifies new metabolites from a Cordyceps ...

Objectives: This study aims to identify new anti‐cancer agents from Cordyceps‐colonizing fungi, using an ecology‐based approach.It also aims to explore their anti‐cell proliferative mechanisms, and to evaluate their anti‐tumour effects in vivo.. Materials and methods: Extracts from Cordyceps‐colonizing fungi were tested on HeLa cells, and active extracts were separated to obtain ...

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Plant Tissue Culture

Practically any plant transformation experiment relies on tissue culture. At HiMedia a complete range of plant tissue culture products are offered that facilitate protocol standardization for mass multiplication and developing new traits of the plant using modern biotechnology.

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Research | Discovery of Anticancer Agents of Diverse Natural Origin - U.OSU

Biological screening is offered for Projects 1-3 using a selection of secondary cell-based and mechanism-based assays (Dr. Esperanza Carcache de Blanco). Project 2 - Isolation Chemistry of Cultured Cyanobacteria and Plant Acquisition [University of Illinois at Chicago (UIC); Project Leader, Dr. Jimmy Orjala].

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In vitro screening antiviral activity of Thai medicinal plants against ...

Using antiviral screening, we observed that T. triandra extract strongly inhibited PRRSV infectivity in MARC-145 cells [virus titer 3.5 median tissue culture infective dose (TCID 50 )/ml (log10)] at 24 h post-infection, whereas C. sappan extract strongly inhibited PRRSV replication [virus titer 2.5 TCID 50 /ml (log10)] at 72 h post-infection.

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Screening of Venezuelan Medicinal Plant Extracts for Cytostatic and ...

Screening of Venezuelan Medicinal Plant Extracts for Cytostatic and Cytotoxic Activity Against Tumor Cell Lines ... Appraisal of Medicinal Plants with Anticancer Properties in South America, Anticancer ... E. Pagani, J. de F.L. Santos, E. Rodrigues, Culture-Bound Syndromes of a Brazilian Amazon Riverine population: Tentative correspondence ...

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Secondary Metabolites of Solanaceae and their Anti-Cancer Potential: An ...

Many plants belonging to the Solanaceae family have been used as a source of pharmaceuticals for centuries due to the presence of tropane and nicotine alkaloids, as principle actives [92-95]. Some Solanum-genera plants have traditionally been used as anti-cancer and anti-herpes agents from olden times [96-100].

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A new extract of the plant calendula officinalis produces a dual in ...

Plants have a long history of use in the treatment of cancer. Active principles of Angelica Gigas, Catharanthus roseus, Podophyllum peltatum, Podophyllum emodii, Taxus brevifolia, Ocrosia elliptica, and Campototheca acuminata have been used in the treatment of advanced stages of various malignancies in the clinical setting [1, 2].Furthermore, many phytochemicals with different pharmacological ...

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Screening of solanum nigrum for its phytochemical and antimicrobial ...

pure culture. All the bacteria were isolated and identified using morphological and biochemical tests adopting standard procedures (Cowan and Steel, 1974). Stock culture was maintained in both Agar slant and 20% sterile buffered glycerol. Phytochemical Screening The plant extracts were screened for the presence

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D Evaluation of biological activities of Physalis peruviana ... - CORE

Besides this plant is known to induce apoptosis in different phases and has anti-cancer activity (Yen et al., 2010). Therefore, the interest in this genus has grown in many regions of the world in recent decade. In this study we aimed to examine the antioxidant and antibacterial activities of potential anticancer plant, Physalis

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Anticancer Activity of Ipomoea purpurea Leaves Extracts in ... - Hindawi

Anticancer Activity of Ipomoea purpurea Leaves Extracts in Monolayer and Three-Dimensional Cell Culture: Cancer is a leading cause of death and a vital health care challenge in the world. Hence, this work was conducted to determine the in vitro anticancer property and also the molecular mechanism of aqueous and organic extracts of >Ipomoea purpurea leaves in three human cancer cell lines ...

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Cytotoxicity of methanol extracts of Annona muricata, Passiflora edulis ...

Cancer cells rapidly acquire resistance leading to treatment failures. In the present study, we have evaluated the cytotoxicity of 17 methanol extracts from 11 Cameroonian medicinal plants against the sensitive leukemia CCRF-CEM cells and the best ones were further tested on a panel of 8 other human cancer cell lines, including various MDR phenotypes as well as against the normal AML12 ...

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Extraction, Isolation and Characterization of Bioactive Compounds from ...

The premier steps to utilize the biologically active compound from plant resources are extraction, pharmacological screening, isolation and characterization of bioactive compound, toxicological evaluation and clinical evaluation. A brief summary of the general approaches in extraction, isolation and characterization of bioactive compound from ...

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In Vitro Screening for Cytotoxic Activity of Herbal Extracts - Hindawi

In the present study, we performed an in vitro screening of ethanol extracts from commercial plants in order to investigate potential antitumor activity against human tumor cell lines. Experimental results obtained through a variety of methods and techniques indicated that extracts of I. verum, G. glabra, R. Frangula, and L. usitatissimum ...

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Antioxidant, Antimicrobial, and Anticancer Effects of Anacardium Plants ...

Anacardium plants have received increasing recognition due to its nutritional and biological properties. A number of secondary metabolites are present in its leaves, fruits, and other parts of the plant. Among the diverse Anacardium plants' bioactive effects, their antioxidant, antimicrobial, and anticancer activities comprise those that have gained more attention. Thus, the present article ...

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Phytochemical Screening and Antifungal Activity of Semecarpus ...

The spread of antibiotic resistant pathogens is one of the most serious menaces to successful treatment of microbial diseases. Medicinal and aromatic plants are widely used as traditional medicines and constitute a major source of natural organic compounds. Semecarpus anacardium Linn (Family: Anacardium), is a plant well known for its medicinal value in Ayurvedic and Siddha system of medicine ...

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Anticancer potential of rhizome extract and a labdane ... - Nature

Here, we report for the first time, the anticancer potential of petroleum ether extract from C. mutabilis rhizome (CMRP) and a novel labdane diterpenoid, (E)-14, 15-epoxylabda-8(17), 12-dien-16-al ...

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Metabolic profiling, antioxidant capacity and in vitro anticancer ...

including anticancer activitie (Harlev et al., 2012)s . The aim of this study was to assess the metabolic profiling, antioxidant and anticancer activity of ome plants that s belong to family Compositae that grow widely inthe northern region of KSA. MATERIALS AND METHODS . Collection of plant materials and preparation of plant extracts

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Cytotoxic Properties of Some Medicinal Plant Extracts from Mazandaran, Iran

microbial infection. Cytotoxic screening of plants is the preliminary methods to identify active compounds of plants (3, 4). 2. Objectives In the course of our screening studies for the anticancer compounds from plants, we performed the present study to evaluate the in vitro cytotoxic activity of four plant ex-

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Chromatographic Fractionation of An Ethanolic Extract of Peels from ...

In this current work, the n-hexane fraction from an ethanolic extract of pulverized peel of Ipomoea batatas Lam was subjected to chromatographic fractionation. Consequently, the fractions were investigated for their anticancer potential using the Sulforhodamine-B assay. Chromatographic fractionation led to n-hexane sub-fractions with greater anticancer potential.

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Cristina P R Xavier - Academia.edu

3D Cell Culture Models as Recapitulators of the Tumor Microenvironment for the Screening of Anti-Cancer Drugs more by Cristina P R Xavier Today, innovative three-dimensional (3D) cell culture models have been proposed as viable and biomimetic alternatives for initial drug screening, allowing the improvement of the efficiency of drug development.

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Ethno-medicinal value of some anticancer medicinal plants from north ...

animals. Medicinal plants are used either alone or in combination with another. All these information generated interest for the evaluation of traditional anticancer medicinal plants from this region to ascertain their anti-cancer activity against a murine experimental tumour system as well as screening of phyto-

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Anticancer and Antifolate Activities of Extracts of Six Saudi Arabian ...

Anticancer and Antifolate Activities of Extracts of Six Saudi Arabian Wild Plants Used in Folk Medicine 335 bacterial infections [8]. The six plants selected for the present study are listed in ...

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Phytochemical screening, metabolite profiling and enhanced ...

Microalgae is one of the major sources of natural compounds with antimicrobial activity. The metabolite profiling of the extracts could identify the bioactive compounds based on methanol (MET), ethanol (ETH), chloroform (CHL), hexane (HEX) and water (W) solvent systems. The microalgal crude extracts in co-application with silver nanoparticles (AgNPs) had enhanced antimicrobial activity with ...

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A Review- Anti-Cancer Compounds from Medicinal Plants: Isolation ...

Medicinal Plants: Their Use in Anticancer Treatment. Int. J. Pharm. Journal of Scientific Research 6(10) ... Induction of apoptosis by in vitro and in vivo plant extracts derived from Menyanthes trifoliata L. in human cancer cells. ... Plant Cell, Tissue and Organ Culture 42(1), 1-25. Kluwer Academic Publishers.

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Thieme E-Books & E-Journals - Planta Medica / Issue

8th Joint Meeting of AFERP, ASP, GA, PSE and SIF. New York City, July 28 - August 1, 2012. Chairmen: Guy T. Carter, Scientific Organizing Committee - Edward Kennelly, Local Organizing Committee - Issue Editor: Mark O'Neil-Johnson. Abstracts (HTML) List of Authors. Poster. Topic I: Natural Products Discovery: Marine, Microbes, Plants and ...

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In vitro antimitotic and cytotoxic potential of plant extracts: a ...

Plants have been used in alternative and traditional medicines for the cure of different types of diseases since ancient time. Secondary metabolites from natural sources play a crucial role in the treatment of various ailments. The present study carried out to investigate the phytochemical, antimitotic and cytotoxic activity of methanolic (95%) extracts of Mucuna pruriens seeds, Asteracantha ...

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Screening for Selective Anticancer Activity of 65 Extracts of Plants ...

Finding cytotoxic drugs with a high selectivity towards cancer cells is crucial to improve the low survival rates of patients diagnosed with metastatic cancers. Since plants are an important source of anticancer drugs, we have screened 65 extracts from 45 plants collected in several areas of Western Andalusia (Spain) for cytotoxic activity on lung cancer cells versus lung normal cells. An ...

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High-content screening of Thai medicinal plants reveals ... - Nature

Subsequently, we performed the high-content screening of Thai natural products, consisting of medicinal plant extracts and phytochemical compounds, to search for the new and promising anti-SARs ...

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A REVIEW ON: PLANTS AS ANTICANCER | PharmaTutor

Graviola, is fruit, having natural anti-cancer properties. Graviola (Annona muricata) is an evergreen tree that is used as a remedy for coughs, bacterial and parasite infections, herpes, and arthritis. The extract of Graviola, shows anticancer activity on PC cells.

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Evaluating Medicinal Plants for Anticancer Activity - Hindawi

With a long history of traditional use spanning many centuries, the medicinal plants of Israel present a unique opportunity for focused screening based on their ethnobotanical use. In the current study we initially examined the effects of 17 whole plant extracts (ethanol extraction) of Israeli plants on human tumor cell lines as well as human primary cancer cultures.

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Antimicrobial, antibiofilm, antioxidant, anticancer, and phytochemical ...

Ethyl acetate seed extract of Pongamia pinnata displayed the highest antimicrobial potential against all test pathogens and Staphylococcus epidermidis was reported as the most sensitive strain with MIC/MBC 1.56/3.12 mg ml−1. It inhibited S. epidermidis biofilm 97.43% at MIC and LM as well as FE-SEM micrographs displayed extensive disintegration in biofilm. It showed the highest TPC (1.23 ± ...

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